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US 8,329,438 B2Granted
Enone reductases
AssigneeCodexis, Inc.
InventorsChristopher Savile, Vesna Mitchell, Xiyun Zhang, Gjalt Huisman
Life of this patent
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Abstract
The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
Claims14
An isolated enone reductase polypeptide comprising an amino acid sequence that is at least 80% identical to a reference sequence set forth in SEQ ID:
- 8, wherein the amino acid sequence comprises one or more residue differences as compared to the reference sequence, wherein the residue corresponding to X117 is a cysteine or nonpolar, aliphatic, basic, acidic, polar, or aromatic residue, selected from C, L, A, M, V, I, N, Q, E, F, and S, wherein said enone reductase polypeptide has enone reductase activity.
Dependencies are inferred from the claim text.