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US 8,324,149 B2Granted
Encapsidation of heterologous entities into virus-like particles
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsBradley C. Bundy, James R. Swartz, Wei Chan
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Abstract
Methods are provided for the utilization of bacterial cell-free extracts in the synthesis of high yields of virus like particles with encapsidated cargo.
Claims10
A method for synthesis of virus like particles comprising encapsidated cargo in a cell-free in vitro reaction, the method comprising:
- synthesizing virus coat proteins in a prokaryotic cell-free in vitro translation reaction in the presence of heterologous cargo;
- wherein the virus coat proteins self-assemble into a stable virus like particle comprising at least 60 separate proteins;
- which VLP is free of a viral genome and comprises encapsidated metallic nanoparticle imaging agent.
A method for synthesis of virus like particles comprising encapsidated cargo in a cell-free in vitro reaction, the method comprising:
- synthesizing virus coat proteins in a prokaryotic cell-free in vitro translation reaction in the presence of heterologous cargo wherein said prokaryotic cell-free in vitro translation reaction comprises exogenous translation initiation factors;
- wherein the virus coat proteins self-assemble into a stable virus like particle comprising at least 60 separate proteins;
- which VLP is free of a viral genome and comprises encapsidated heterologous cargo.
Dependencies are inferred from the claim text.