Methods and compositions for producing hydrocarbons
AssigneeLS9, Inc.
InventorsAndreas Schirmer, Mathew Rude, Shane Brubaker
Life of this patent
Estimating the term…
Abstract
Compositions and methods for producing aldehydes, alkanes, and alkenes are described herein. The aldehydes, alkanes, and alkenes can be used in biofuels.
Claims12
A method of producing an alkane or alkene from an engineered microorganism, the method comprising,
- expressing a first gene encoding an acyl-ACP reductase that catalyzes the conversion of acyl ACP to a fatty aldehyde in said engineered microorganism;
- expressing a second gene encoding a decarbonylase that catalyzes the conversion of a fatty aldehyde to an alkane or alkene in said engineered microorganism;
- culturing said engineered microorganism in a culture media containing a carbohydrate carbon source under conditions effective to produce an alkane or alkene in the cell-free supernatant.
A cell culture comprising an engineered microorganism wherein an acyl-ACP reductase that catalyzes the conversion of acyl ACP to a fatty aldehyde and a decarbonylase that catalyzes the conversion of a fatty aldehyde to an alkane or alkene are overexpressed in said engineered microorganism and an alkane or alkene is produced in the cell culture when said engineered microorganism is cultured in medium containing a carbon source under conditions effective to express said acyl-ACP reductase and said decarbonylase.
Dependencies are inferred from the claim text.