Back to results
US 8,323,177 B2Granted
Imaging and evaluating embryos, oocytes, and stem cells
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsConnie C. Wong, Kevin E. Loewke, Thomas M. Baer, Renee A. Reijo-Pera, Barry Behr
Life of this patent
Estimating the term…
Abstract
Methods, compositions and kits for determining the developmental potential of one or more embryos or pluripotent cells and/or the presence of chromosomal abnormalities in one or more embryos or pluripotent cells are provided. These methods, compositions and kits find use in identifying embryos and oocytes in vitro that are most useful in treating infertility in humans.
Claims30
A method for selecting one or more human embryos that is likely to reach the blastocyst stage comprising:
- culturing one or more human embryos under conditions sufficient for embryo development;
- time lapse imaging said one or more embryos for the duration of at least one cytokinesis event or cell cycle;
- measuring at least one cellular parameter comprising:
- (a) the duration of the first cytokinesis;
- or
- (b) the time between the first and second mitosis;
- or
- (c) the time between the second and third mitosis;
- and
- selecting an embryo when said at least one cellular parameter falls within:
- (i) a duration of the first cytokinesis that is about 0 to about 33 minutes;
- or
- (ii) a time between the first and second mitosis that is about 7.8 to 14.3 hours;
- or
- (iii) a time between the second and third mitosis that is about 0 to 5.8 hours;
- thereby selecting an embryo that is more likely to reach the blastocyst stage.
13 more elements
Dependencies are inferred from the claim text.