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US 8,318,486 B2Granted
Co-culturing mammalian embryonic stem cells with human foreskin fibroblasts
AssigneeTechnion Research & Development Foundation Limited
InventorsMichal Amit, Joseph Itskovitz-Eldor
Life of this patent
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Abstract
A cell culture comprising human foreskin cells, the human foreskin cells being capable of maintaining stem cells in an undifferentiated state when co-cultured therewith.
Claims18
A method of maintaining mammalian pluripotent stem cells in an undifferentiated state comprising co-culturing the mammalian pluripotent stem cells with a human foreskin fibroblast feeder cell line, wherein said mammalian pluripotent stem cells are capable of forming teratomas containing cells of the endoderm, mesoderm and ectoderm germ layers.
A cell culture comprising:
- (i) mammalian pluripotent stem cells, wherein said mammalian pluripotent stem cells are capable of forming teratomas containing cells of the endoderm, mesoderm and ectoderm germ layers;
- and
- (ii) human foreskin fibroblast cells capable of maintaining said mammalian pluripotent stem cells in an undifferentiated state.
Dependencies are inferred from the claim text.