Reagent for detecting an analyte
AssigneeMedtronic MiniMed, Inc.
InventorsJens Christian Norrild, Bo Wegge Laursen
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Abstract
A reagent for use in detecting an analyte comprises a fluorescent energy donor and an energy acceptor, wherein the energy acceptor is of the general formula: and wherein the distance between the energy donor and the energy acceptor of the reagent is capable of modulation by a suitable analyte to be detected.
Claims29
A reagent for use in detecting an analyte, comprising a fluorescent energy donor and an energy acceptor, the energy donor and the energy acceptor being such that when they are sufficiently close to one another energy is non-radiatively transferred from the energy donor following excitation thereof to the energy acceptor quenching fluorescence of the energy donor, wherein the energy acceptor is of the formula:
- wherein:
- R1, R2 and R3 are each independently H, electron donating substituents, or electron withdrawing substituents or R3 is attached to a linker structure, provided that at least two of R1, R2 and R3 are electron donating groups;
- R4, R5, R6, R7, R8 and R9 are each independently H, halogen, alkyl, aryl, O-alkyl, S-alkyl and R10,R11, R12, R13, R14 and R15 are each independently hydrogen, O-alkyl, S-alkyl, alkyl, or one or more pairs of groups R1 and R4 and/or R1 and R5 and/or R2 and R6 and/or R2 and R7 and/or R3 and R8 and/or R3 and R9 and/or R4 and R10 and/or R5 and R11 and/or R6 and R12 and/or R7 and R13 and/or R8 and R14 and/or R9 and R15 is a bridging group consisting of aryl, alkylene, O-alkylene, S-alkylene or N-alkylene optionally substituted with one or more of SO3−, PO32−, OH, O-alkyl, SH, S-alkyl, COOH, COO−, ester, amide, halogen, SO-alkyl, SO2-alkyl, SO2NH2, SO2NH-alkyl, SO2N-dialkyl, SO3-alkyl, CN, secondary amine or tertiary amine, provided that not all of R10, R11, R12, R13, R14 and R15 are hydrogen;
- wherein a linker structure is attached to the energy acceptor at R3, or where a bridging group is present optionally the linker structure is attached to the energy acceptor at the bridging group, the linker structure being formed by reaction of a linker element selected from an active ester, an isothiocyanate, an acid chloride, an aldehyde, an azide, an α-halogenated ketone and an amine with a reaction partner;
- and wherein the distance between the energy donor and the energy acceptor of the reagent is capable of modulation by a suitable analyte to be detected.
A dye compound having the formula:
- wherein:
- R4, R5, R6, R7, R8 and R9 are each independently H, halogen, alkyl, aryl, O-alkyl or S-alkyl and R10, R11, R12, R13, R14 and R15 are each independently hydrogen, O-alkyl, S-alkyl, or alkyl, or one or more pairs of groups R20 and R4 and/or R20 and R5 and/or R4 and R10 and/or R5 and R11 and/or R6 and R12 and/or R7 and R13 and/or R8 and R14 and/or R9 and R15 is a bridging group consisting of aryl, alkylene, O-alkylene, S-alkylene or N-alkylene optionally substituted with one or more of SO3−, PO32−, OH, O-alkyl, SH, S-alkyl, COOH, COO−, ester, amide, halogen, SO-alkyl, SO2-alkyl, SO2NH2, SO2NH-alkyl, SO2N-dialkyl, SO3-alkyl, CN, secondary amine or tertiary amine, provided that not all of R10, R11, R12, R13, R14 and R15 are hydrogen;
- R16, R17, R18 and R19 are each independently H, alkyl or aryl, or one or more of R16 and R17 or R18 and R19 is alkylene, optionally substituted with one or more of SO3−, PO32−, OH, O-alkyl, SH, S-alkyl, COOH, COO−, ester, amide, halogen, SO-alkyl, SO2-alkyl, SO2NH2, SO2NH-alkyl, SO2N-dialkyl, SO3-alkyl, CN, secondary amine or tertiary amine;
- or one or more of pairs of groups R6 and R16, R7 and R17, R8 and R18 and R9 and R19 is alkylene, O-alkylene, S-alkylene or N-alkylene optionally substituted with one or more of SO3−, PO32−, OH, O-alkyl, SH, S-alkyl, COOH, COO−, ester, amide, halogen, SO-alkyl, SO2-alkyl, SO2NH2, SO2NH-alkyl, SO2N-dialkyl, SO3-alkyl, CN, secondary amine or tertiary amine
- and
- R20 is a linker element selected from an active ester, an isothiocyanate, an acid chloride, an a-halogenated ketone and an azide.
Dependencies are inferred from the claim text.