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US 8,318,421 B2Granted
Hepatitis-C virus testing
AssigneeCommon Services Agency
InventorsPeter Simmonds, Shiu-Wan Chan, Peng Lee Yap
Life of this patent
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Abstract
New styles of hepatitis C virus (HCV), referred to as HCV-3 and HCV-4, have been identified and sequenced. Antigenic regions of HCV-2, HCV-3 and HCV-4 polypeptides have been identified. Immunoassays for HCV and antibodies thereto are described, which allow more complete screening of blood samples for HCV, and allow HCV genotyping.
Claims1
A method for detecting HCV infection in a mammal, comprising:
- a) obtaining a blood sample from a mammal;
- b) isolating RNA from said blood sample;
- c) reverse transcribing said RNA intro cDNA;
- d) performing PCR on said cDNA using sense and antisense primers specific for HCV, wherein said sense and antisense primers are selected from the group consisting of:
- 1) SEQ ID NO:42 and SEQ ID NO:41;
- 2) SEQ ID NO:43 and SEQ ID NO:40;
- and
- 3) SEQ ID NO:43 and SEQ ID NO:41;
- and
- e) determining whether HCV infection was detected by analyzing the results from said PCR.
7 more elements
Dependencies are inferred from the claim text.