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US 8,313,902 B2Granted
Magnetic fine particles having lower critical solution temperature
AssigneeJnc Corporation
InventorsHirotaka Furukawa, Noriyuki Ohnishi, Kazunori Kataoka, Katsuhiko Ueno
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Abstract
The present invention relates to magnetic fine particles having a lower critical solution temperature to which at least one substance selected from biotin and avidin is immobilized, and a method of converting a substance, a method of separating or concentrating a microorganism, a method of modifying a denatured protein, a method of detecting a nucleic acid, a separating agent, and a method of separating a biological substance using the same.
Claims7
A method for converting an objective substance into a converted material and recovering the converted material, comprising
- providing magnetic fine particles having immobilized thereto a copolymer that has a lower critical solution temperature, wherein the copolymer comprises a unit derived from a comonomer containing biotin as a constituent,
- immobilizing a substance having a mutual specific action with an objective substance to the magnetic fine particles to which biotin is bound,
- mixing the resulting magnetic fine particles and the objective substance to create a solution, in which the substance and the objective substance react,
- precipitating the magnetic fine particles by raising the temperature of the solution to a temperature not lower than the lower critical solution temperature of the magnetic fine particles, and
- recovering the converted material,
- wherein the biotin-containing comonomer is a biotin methacrylamide derivative represented by formula (12):
3 more elements
Dependencies are inferred from the claim text.