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US 8,313,897 B2Granted
Method for preserving viral particles
AssigneeStabilitech Ltd.
InventorJeffrey Drew
Life of this patent
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Abstract
A method for preserving viral particles comprises: (i) providing an aqueous solution of one or more sugars, a polyethyleneimine and said viral particles wherein the concentration of polyethyleneimine is 15 μM or less based on the number-average molar mass (Mn) of the polyethyleneimine and the sugar concentration or, if more than one sugar is present, total sugar concentration is greater than 0.1 M; and (ii) drying the solution to form an amorphous solid matrix comprising said viral particles.
Claims13
A method for preserving viral particles comprising:
- (i) providing an aqueous solution of one or more sugars, a polyethyleneimine and said viral particles wherein, (a) the concentration of polyethyleneimine is less than 5 nM based on the number-average molar mass (Mn) of the polyethyleneimine, and (b) said aqueous solution comprises sucrose at a concentration of 0.8M or greater;
- and
- (ii) freeze-drying the solution to form an amorphous solid matrix comprising said viral particles.
A method of preparing a composition comprising viral particles, which method comprises:
- (a) providing an aqueous solution of one or more sugars, a polyethyleneimine and said viral particles, wherein (i) the concentration of polyethyleneimine is less than 5 nM based on the number-average molar mass (Mn) of the polyethyleneimine, and (ii) said aqueous solution comprises sucrose at a concentration of 0.8M or greater;
- (b) adding an adjuvant, buffer, antibiotic and/or additive to the admixture;
- and
- (c) freeze-drying the solution to form an amorphous solid matrix comprising said viral particles.
1 more element
Dependencies are inferred from the claim text.