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US 8,304,250 B2Granted
Multi-dimensional chromatographic methods for separating N-glycans
AssigneeMomenta Pharmaceuticals, Inc.
InventorsIan Christopher Parsons, Ting Zheng, Nur Sibel Gunay, Carlos J. Bosques
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Abstract
A multi-dimensional chromatographic method for the separation of N-glycans. The method comprises providing a glycan preparation that includes at least one negatively charged N-glycan. The glycan preparation is then separated by anion-exchange chromatography and at least one secondary chromatographic technique.
Claims16
A method of characterizing a mixture of N-glycans, said method comprising steps of:
- (i) providing a glycan preparation, wherein the glycan preparation includes at least one charged N-glycan and a known quantity of a reference N-glycan, wherein the reference N-glycan is labeled with a labeling agent;
- (ii) separating the glycan preparation by anion-exchange chromatography to generate a plurality of sample fractions;
- (iii) separating a first portion of the plurality of sample fractions by at least one secondary chromatographic technique;
- and
- (iv) separating a second portion of the plurality of sample fractions by at least one secondary chromatographic technique that differs from the secondary chromatographic technique from (iii);
- and
- (v) quantifying at least one N-glycan in the glycan preparation relative to the reference N-glycan.
4 more elements
Dependencies are inferred from the claim text.