Corn seed comprising novel opaque modifiers and related methods
AssigneeMonsanto Technology LLC
InventorsThomas Malvar, Shihshieh Huang, Alessandra Frizzi, Mike Kerns
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Abstract
Novel corn opaque modifier loci, associated molecular markers and methods for obtaining the loci, markers, and resultant seed are provided. Vitreous seed with reduced alpha-zein storage protein content that contain the opaque modifier loci are also described. Processes for obtaining milled corn seed products from the vitreous seed with reduced alpha-zein storage protein content that contain the opaque modifier loci are also provided.
Claims12
A corn seed comprising a vitreous kernel phenotype, at least one genetic element that comprises one or more recombinant DNA(s) encoding one or more transgene(s) that reduce(s) expression of both 19 kilodalton (kD) and 22 kilodalton (kD) alpha-zein storage relative to a control seed, and at least one opaque modifier locus that imparts said vitreous kernel phenotype to said seed comprising said genetic element, wherein a 27 kilodalton gamma zein storage protein content in said corn seed is less than 2-fold higher than that of a seed of the same variety that contains said genetic element but lacks said opaque modifier, and wherein said vitreous seed has a kernel density of at least about 1.24 grams/milliliter, and wherein said opaque modifier comprises at least one genetic locus present in corn line I226211 germplasm deposited as seed under ATCC Accession No. PTA-8214 or wherein said opaque modifier comprises at least one genetic locus present in corn line I283669 germplasm deposited as seed under ATCC Accession No. PTA-8569.
Dependencies are inferred from the claim text.