RNA molecules and vectors for gene silencing
AssigneePlant Bioscience Limited
InventorsDavid Charles Baulcombe, Andrew John Hamilton
Life of this patent
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Abstract
The invention is directed to compositions for gene silencing by providing short RNA molecules to cells.
Claims15
A composition for introduction into a cell to effect gene silencing, consisting essentially of isolated short antisense RNA molecules (SARMs) and isolated short sense RNA molecules (SSRMs), collectively short RNA molecules (SRMs),
- wherein the SSRMs and the SARMs consist of 21-30 nucleotides;
- wherein said SARMS are complementary to, and can base pair with, a target RNA, which target RNA is transcribed from a gene that is silenced when said SRMs are present in a cell containing said gene, and said SSRMs correspond to the target RNA;
- and
- wherein said gene is endogenous to an organism selected from the group consisting of a plant, a mammal, an avian organism, a reptile, an insect, and a protozoan, or
- said target RNA is generated by a pathogen.
A composition for introduction into a cell to effect gene silencing, which composition comprises at least one vector which, when introduced into a cell, produces short antisense RNA molecules (SARMs) and short sense RNA molecules (SSRMs), said SARMs and SSRMs designated, collectively, short RNA molecules (SRMs),
- wherein the SSRMs and SARMs consist of 20-30 nucleotides;
- wherein said SARMs are complementary to, and can base pair with, a target RNA, which target RNA is transcribed from a gene that is silenced when said SRMs are present in a cell containing said gene, and said SSRMs correspond to the target RNA;
- wherein said gene is endogenous to an organism selected from the group consisting of a plant, a mammal, an avian organism, a reptile, an insect, and a protozoan, or
- said target RNA is generated by a pathogen.
Dependencies are inferred from the claim text.