siRNA hat inhibits WT1 gene expression and uses thereof
AssigneeHaruo Sugiyama
InventorsHaruo Sugiyama, Yusuke Oji
Life of this patent
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Abstract
The present inventors discovered that siRNAs targeting the 17AA site of the WT1 gene not only suppress the expression of the WT1 gene, but also demonstrate remarkable cell growth-suppressing effects and cell death-inducing effects in cancer cell lines.
Claims27
An siRNA molecule comprising a sense strand hybridized to an antisense strand, wherein the antisense strand targets a region in a 17AA site of a Wilms' tumor gene transcript, the siRNA suppresses cell growth, the sense strand consists of SEQ ID NO:
- 1 plus an optional single-stranded overhang, and the antisense strand consists of SEQ ID NO:
- 2 plus an optional single-stranded overhang.
A DNA comprising a sequence that is transcribed into a sense RNA strand and an antisense RNA strand that hybridize together to form an siRNA that suppresses cell growth, wherein the antisense RNA strand targets a region in a 17AA site of a Wilms' tumor gene transcript, the sense RNA strand consists of SEQ ID NO:
- 1 plus an optional single-stranded overhang, and the antisense strand consists of SEQ ID NO:
- 2 plus an optional single-stranded overhang.
A pair of DNAs, the first DNA comprising a sequence that is transcribed into a sense RNA strand and the second DNA comprising a sequence that is transcribed into an antisense RNA strand, wherein the sense and antisense RNA strands hybridize together to form an siRNA that suppresses cell growth, the antisense RNA strand targets a region in a 17AA site of a Wilms' tumor gene transcript, the sense RNA strand consists of SEQ ID NO:
- 1 plus an optional single-stranded overhang, and the antisense strand consists of comprises SEQ ID NO:
- 2 plus an optional single-stranded overhang.
An isolated RNA consisting of an RNA sequence that is the RNA equivalent of the sequence set forth in SEQ ID NO:
- 3.
An isolated DNA consisting of the sequence set forth in SEQ ID NO:
- 3 and optionally one or more transcription control sequences operably linked to the sequence set forth in SEQ ID NO:
- 3.
An isolated RNA consisting of:
- (i) an RNA sequence that is the RNA equivalent of the sequence set forth in SEQ ID NO:
- 3 and
- (ii) one to eight overhanging nucleotides at either or both of the 5′ and 3′ termini of the sequence of (i).
An isolated DNA having a strand consisting of:
- (i) the sequence set forth in SEQ ID NO:
- 3;
- and
- (ii) one to eight additional nucleotides at either or both of the 5′ and 3′ termini of (i), wherein the strand optionally includes one or more transcription control sequences.
A vector that expresses an RNA consisting of an RNA sequence that is the RNA equivalent of the sequence set forth in SEQ ID NO:
- 3.
A vector that expresses an RNA molecule consisting of:
- (i) an RNA sequence that is the RNA equivalent of the sequence set forth in SEQ ID NO:
- 3, and
- (ii) one to eight overhanging nucleotides at either or both of the 5′ and 3′ termini of the sequence of (i).
Dependencies are inferred from the claim text.