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AssigneesDepartment of Biotechnology, All India Institute of Medical Science
InventorsSarman Singh, Ayan Dey
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A novel recombinant chimera of DNA construct having esat-6 region of Mycobacterium tuberculosis and kinesin region of Leishmania donovani cloned together on two sides of self cleaving peptide in a DNA vaccine vector pVAX-1 wherein the chimeric construct is operatively linked to a transcriptional promoter thus capable of self replication and expression within the mammalian cell, and the process of preparation thereof comprising: analysis of the predicted protein sequence of kinesin motor domain and esat-6 domain using Promiscuous MHC Class-1 Binding Peptide Prediction Servers; amplification of gene coding for kinesin motor domain and esat-6 domain; cloning of kinesin esat-6 gene region in pGEM-T™ vector for sequence analysis; generation of chimeric construct by directional cloning in pVAX-1 vector. In-vitro expression analysis of kinesin motor domain and esat-6 domain from the clones using cell free translation system and immunogenicity studies; and splenocyte proliferation and cytokines studies using the above mentioned constructs.
A novel recombinant chimera of DNA construct comprising an esat-6 region of Mycobacterium tuberculosis and a kinesin region of Leishmania donovani cloned together on two sides of a self cleaving peptide in a DNA vaccine vector pVAX-1 wherein the chimeric construct is operatively linked to a transcriptional promoter and is capable of self replication and expression within a mammalian cell.
A pharmaceutically acceptable immune stimulatory preparation comprising a DNA sequence of a kinesin motor domain from Leishmania donovani and an esat-6 domain from Mycobacterium tuberculosis.
A method of preparing a recombinant chimera of DNA construct comprising:
Dependencies are inferred from the claim text.