High-purity Rebaudioside D

A method for purifying Rebaudioside D from Stevia extract comprising the steps of:

• a. providing an extract of Stevia rebaudiana Bertoni plant;
• b. dissolving the extract in a first aqueous solution of organic solvent to result in a first mixture of steviol glycosides, wherein the organic solvent is selected from the group consisting of methanol, ethanol, l-propanol, isopropanol, and a mixture thereof, and the organic solvent is 75-99 vol. %;
• c. inducing crystallization in the first mixture;
• d. filtering the mixture from step (c) to obtain a first precipitate and a first filtrate;
• e. dissolving the first precipitate in a second aqueous solution of organic solvent to result in a second mixture, wherein the organic solvent is selected from the group consisting of methanol, ethanol, l-propanol, isopropanol, and a mixture thereof, and the organic solvent is 70-80-vol. %;
• f. inducing crystallization in the second mixture;
• g. filtering the mixture from step (f) to obtain a second precipitate and a second filtrate;
• h. dissolving the second precipitate in a third aqueous solution of organic solvent to result in a third mixture, wherein the organic solvent is selected from the group consisting of methanol, ethanol, l-propanol, isopropanol, and a mixture thereof, and the organic solvent is 10-80 vol. %;
• i. inducing crystallization in the third mixture;
• and
• j. filtering the mixture from step (i) to obtain a third precipitate and a third filtrate;
• whereby drying the third precipitate yields purified Rebaudioside D.