Variant AXMI-R1 delta endotoxin genes and methods for their use
AssigneeAthenix Corporation
InventorVolker Heinrichs
Life of this patent
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Abstract
Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, nucleic acid molecules encoding variant AXMI-R1 sequences are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed.
Claims12
A recombinant polypeptide comprising a variant Cry3 amino acid sequence, wherein said variant comprises one or more amino acid substitutions in the amino acid sequence of SEQ ID NO:2, and wherein the one or more amino acid substitutions are selected from the group consisting of:
- a) an alanine, glutamine, or histidine residue at the position 154 of SEQ ID NO:2;
- b) a glutamic acid or a lysine residue at the position 155 of SEQ ID NO:2;
- c) a glycine residue at the position 158 of SEQ ID NO:2;
- d) an alanine, isoleucine, leucine, phenylalanine, threonine, or valine residue at the position 160 of SEQ ID NO:2;
- e) a methionine or tryptophan residue at the position 315 of SEQ ID NO:2;
- f) an alanine, glutamine, glutamic acid, or threonine residue at the position 316 of SEQ ID NO:2;
- g) an isoleucine or leucine residue at the position 482 of SEQ ID NO:2;
- h) a glutamine, lysine, or serine residue at the position 483 of SEQ ID NO:2;
- and
- i) an asparagine residue at the position 519 of SEQ ID NO:2;
- and wherein said variant has pesticidal activity.
Dependencies are inferred from the claim text.