Antimicrobial peptides and methods of their use
AssigneeThe Ohio State University Research Foundation
InventorsAhmed El-Meleigy Yousef, Zengguo He, Chunhua Yuan, Liwen Zhang, Duygu Kisla
Life of this patent
Estimating the term…
Abstract
The present invention relates to a novel Paenibacillus polymyxa strain, OSY-DF, and its bioactive mutants. Also provided is a method for using a novel antimicrobial peptide, paenibacillin, isolated from the bacterial strain OSY-DF, and its bioactive variants or fragments. The invention also relates to antimicrobial compositions containing same and methods of their use.
Claims19
An isolated peptide comprising an amino acid sequence that is at least 80% identical to the sequence SEQ ID NO.5, wherein the sequence comprises:
- (i) amino acids at positions 1, 6, 7, 11, 15, 17, 19, 20, 22, 23, 25, 26, and 29 that are identical to the amino acids in SEQ ID NO.5;
- (ii) at least four Lys residues wherein at least one of said Lys residues is at position 30;
- (iii) one or more dehydrated amino acids α,β-dehydro-alanine (Dha);
- (iv) one or more dehydrated amino acids α,β-dehydro-butyrine (Dhb);
- and
- (v) a Dhb-Dhb tandem,
- wherein the peptide is a lantibiotic.
A method of preventing or treating a Gram-positive bacterial infection in a subject comprising administering to said subject an effective amount of a composition comprising a peptide, wherein said peptide is a lantibiotic comprising an amino acid sequence that is at least 80% identical to the sequence SEQ ID NO.5, wherein the sequence comprises:
- (i) amino acids at positions 1, 6, 7, 11, 15, 17, 19, 20, 22, 23, 25, 26, and 29 that are identical to the amino acids in SEQ ID NO.5;
- (ii) at least four Lys residues wherein at least one of said Lys residues is at position 30;
- (iii) one or more dehydrated amino acids a,,6-dehydro-alanine (Dha);
- (iv) one or more dehydrated amino acids a,,6-dehydro-butyrine (Dhb);
- and
- (v) a Dhb-Dhb tandem.
A method for preventing or inhibiting the growth of Gram-positive bacteria on or inside an environment, comprising contacting said environment with an effective amount of a composition comprising a peptide;
- wherein said peptide is a lantibiotic comprising an amino acid sequence that is at least 80% identical to the sequence SEQ ID NO.5, wherein the sequence comprises:
- (i) amino acids at positions 1, 6, 7, 11, 15, 17, 19, 20, 22, 23, 25, 26, and 29 that are identical to the amino acids in SEQ ID NO.5;
- (ii) at least four Lys residues wherein at least one of said Lys residues is at position 30;
- (iii) one or more dehydrated amino acids α,β-dehydro-alanine (Dha);
- (iv) one or more dehydrated amino acids α,β-dehydro-butyrine (Dhb);
- and
- (v) a Dhb-Dhb tandem.
Dependencies are inferred from the claim text.