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US 8,298,995 B2Granted
Repeatable protein arrays
AssigneeThe Babraham Institute
InventorsMichael John Taussig, Mingyue He
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Abstract
The invention relates to a method of producing a protein array (5) on one support surface (3) from a corresponding nucleic acid array on a separate surface (1), to protein arrays produced by the method, to uses of the protein arrays in the identification of interactions between arrayed proteins and other molecules, and to kits for producing said protein arrays.
Claims15
A method of producing a protein array, comprising:
- a) exposing a nucleic acid array immobilized on a first support surface to a cell-free system wherein the cell-free system is capable of performing protein synthesis by transcription and translation;
- b) expressing a plurality of proteins from said nucleic acid array;
- and
- c) immobilizing said proteins as a corresponding array on a second support surface, characterized in that said second support surface is directly in contact with said first support surface or said first and second support surfaces are configured to be of such proximity to ensure that the proteins are immobilized on said second support surface as they are formed;
- wherein the first and second support surfaces are selected from the group consisting of a slide and a membrane.
2 more elements
Dependencies are inferred from the claim text.