Methods of evaluating a test agent in a diseased cell model

A method of evaluating a test agent in an in vitro diseased cell model, said method comprising:

• a) co-culturing epithelial and/or endothelial cells with a lymphoid tissue in the presence of a test agent;
• b) transferring cells, culture media, or cells and culture media, from the co-culture of a) to a culture of diseased cells, wherein said diseased cells are immune system cells selected from the group consisting of PBMCs, monocytes, macrophages, dendritic cells, lymphocytes and antigen-presenting cells;
• and
• c) evaluating an effect of the test agent on the diseased cells in the culture of b), thereby evaluating a test agent in an in vitro diseased cell model.

A method of evaluating a test agent in an in vitro diseased cell model, said method comprising:

• a) priming a culture of epithelial and/or endothelial cells with a test agent;
• b) transferring cells from the culture of a) to a culture of a lymphoid tissue;
• c) transferring cells, culture media, or cells and culture media from the culture of b) to a culture of diseased cells, wherein said diseased cells are immune system cells selected from the group consisting of PBMCs, monocytes, macrophages, dendritic cells, lymphocytes and antigen-presenting cells;
• and
• d) evaluating an effect of the test agent on the diseased cells in the culture of c), thereby evaluating a test agent in an in vitro diseased cell model.