Cosmid vector for plant transformation and use thereof

A cosmid vector having a full length of 15 kb or less characterized in that:

• 1) it contains an origin of replication (oriV) of an IncP plasmid but does not contain any origin of replication of other plasmid groups;
• 2) it contains the trfA1 gene of an IncP plasmid;
• 3) it contains an origin of conjugative transfer (oriT) of an IncP plasmid;
• 4) it contains the incC1 gene of an IncP plasmid;
• 5) it contains a cos site of lambda phage and the cos site is located outside the T-DNA;
• 6) it contains a drug resistance gene expressed in E. coli and a bacterium of the genus Agrobacterium;
• 7) it contains a T-DNA right border sequence of a bacterium of the genus Agrobacterium;
• 8) it contains a T-DNA left border sequence of a bacterium of the genus Agrobacterium;
• 9) it contains a selectable marker gene for plant transformation located between 7) and 8) and expressed in a plant;
• and
• 10) it contains restriction endonuclease recognition site(s) located between 7) and 8) for cloning a foreign gene,
• wherein the cosmid vector is selected from the group consisting of:
• the cosmid vector pLC40 consisting of the nucleotide sequence of SEQ ID NO:
• 2;
• the cosmid vector pLC40GWH consisting of the nucleotide sequence of SEQ ID NO:
• 3;
• the cosmid vector pLC40 bar consisting of the nucleotide sequence of SEQ ID NO:
• 4;
• the cosmid vector pLC40GWB consisting of the nucleotide sequence of SEQ ID NO:
• 5;
• the cosmid vector pLC40GWHKorB consisting of the nucleotide sequence of SEQ ID NO:
• 65;
• the cosmid vector pLCleo consisting of the nucleotide sequence of SEQ ID NO:
• 66;
• and
• the cosmid vector pLC40GWHvG1 consisting of the nucleotide sequence of SEQ ID NO:
• 7.