Expression systems using mammalian beta-actin promoter
AssigneeChugai Seiyaku Kabushiki Kaisha
InventorsHiroyuki Tsunoda, Kiyoshi Habu
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Abstract
Promoter activities were examined by comparing combinations of promoters and enhancers derived from various genes. A hybrid promoter comprising a combination of a CMV enhancer and a mammalian β-actin promoter, or the post-transcriptional regulatory region of the genomic sequence Woodchuck Hepatitis Virus (WPRE) and a mammalian β-actin promoter was found to be stronger than existing promoters. Furthermore, the activities of the β-actin promoters could be enhanced by coexpressing the oncogene product Ras, which is a transactivator.
Claims34
A DNA construct comprising a mouse β-actin promoter operably linked to a human Cytomegalovirus (CMV) enhancer.
A DNA construct comprising a mouse β-actin promoter operably linked to a CMV enhancer, wherein the CMV enhancer comprises the nucleotide sequence shown in SEQ ID NO:
- 4 and the mouse β-actin promoter comprises the nucleotide sequence shown in SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.