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US 8,298,812 B2Granted
Use of Saccharomyces cerevisiae erg4 mutants for expressing mammalian glucose transporters
AssigneeSanofi-Aventis Deutschland GmbH
InventorsGuenter Mueller, Silke Dlugai, Doerthe Voss, Eckhard Boles
Life of this patent
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Abstract
The invention relates to yeast strains in which a human GLUT4 transport or a human GLUT1 transporter can be functionally expressed and to particular GLUT4 transport proteins which can be functionally expressed particularly readily in yeast strains.
Claims3
An engineered strain of Saccharomyces cerevisiae
- (a) which can no longer grow on substrates with hexoses as the only carbon source,
- (b) which lacks a functional protein expressed from Saccharomyces cerevisiae open reading frame YMR212c (Fgy1) protein, and
- (c) which further lacks a functional sterol C-24(28)-reductase (Erg4) protein, whose ability of growing on a substrate with a hexose as the only carbon source is restored when a glucose transporter type 4 (GLUT4) gene is expressed in this strain, wherein the GLUT4 gene encodes an amino acid sequence comprising SEQ ID NO:2, wherein the methionine residue of position 85 is a valine residue.
Dependencies are inferred from the claim text.