Production of peracids using an enzyme having perhydrolysis activity
AssigneeE.I. du Pont de Nemours and Company
InventorsRobert DiCosimo, John Edward Gavagan, Mark Scott Payne, Frederick B. Cooling
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Abstract
A process is provided for producing peroxycarboxylic acids from carboxylic acid esters. More specifically, carboxylic acid esters are reacted with an inorganic peroxide, such as hydrogen peroxide, in the presence of an enzyme catalyst having perhydrolysis activity. The present perhydrolase catalysts are classified as members of the carbohydrate esterase family 7 (CE-7) based on the conserved structural features. Further, disinfectant formulations comprising the peracids produced by the processes described herein are provided.
Claims5
A recombinant Escherichia coli cell comprising a disruption in katE and a disruption in katG with the proviso that the host cell is not Escherichia coli UM2, wherein said cell further comprises at least one enzyme having perhydrolase activity and a signature motif that aligns with a reference sequence SEQ ID NO:
- 2 using CLUSTALW, said signature motif comprising:
- i) an RGQ motif at positions corresponding to positions 118-120 of SEQ ID NO:2;
- ii) a GXSQG motif at positions corresponding to positions 179-183 of SEQ ID NO:2;
- and
- iii) an HE motif at positions corresponding to positions 298-299 of SEQ ID NO:2.
Dependencies are inferred from the claim text.