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US 8,298,803 B2Granted
Polypeptides having arabinofuranosidase activity and polynucleotides encoding same
AssigneeNovozymes A/S
InventorsMichelle Maranta, Kimberly Brown, James Langston
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Abstract
The present invention relates to isolated polypeptides having alpha-L-arabinofuranosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Claims13
An isolated polynucleotide encoding a polypeptide having alpha-L-arabinofuranosidase activity, selected from the group consisting of:
- (a) a polynucleotide encoding a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
- 2;
- (b) a polynucleotide that hybridizes under at least high stringency conditions with the mature polypeptide coding sequence of SEQ ID NO:
- 1 or the cDNA sequence thereof, or the full-length complementary strand thereof, wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide, and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.;
- and
- (c) a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 1 or the cDNA sequence thereof.
4 more elements
Dependencies are inferred from the claim text.