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US 8,298,800 B2Granted
Lipolytic enzyme variants
AssigneeNovozymes A/S
InventorsKim Borch, Luise Erlandsen, Jesper Vind, Allan Svendsen, Christel Thea Jorgensen
Life of this patent
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Abstract
The inventors have developed improved polypeptides by substituting or deleting specified amino acids in fungal lipolytic enzymes. More particularly, the polypeptides result in a reduction of dough stickiness when they are added to a dough. The polypeptides may particularly have activity on polar lipids.
Claims14
A variant polypeptide which:
- (a) has hydrolytic activity towards an ester bond in a polar lipid, and
- (b) has an amino acid sequence which
- (i) has at least 80% identity to SEQ ID NO:
- 1 and has a different amino acid or an amino acid deletion at a position corresponding to A29, K33, I83 or A255, or
- (ii) has at least 80% identity to SEQ ID NO:
- 2 and has a different amino acid or an amino acid deletion at a position corresponding to R84 or P256.
3 more elements
Dependencies are inferred from the claim text.