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US 8,298,798 B2Granted
Production of C5-C8 alcohols using evolved enzymes and metabolically engineered microorganisms
AssigneeThe Regents of the University of California
InventorsJames C. Liao, Kechun Zhang
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Abstract
Provided herein are metabolically-modified microorganisms useful for producing biofuels. More specifically, provided herein are methods of producing higher alcohols including C5-C8 alcohol from a suitable substrate.
Claims2
A purified polypeptide consisting of SEQ ID NO:2, having a mutation in any of the following residues D430, A453, A460, or G462 to remove leucine feedback inhibition, and comprising an additional mutation consisting of S139G, wherein the polypeptide initiates the conversion of 2-keto-3-methylvalerate to a C6-C9 keto acid.
Dependencies are inferred from the claim text.