Cinnamyl-alcohol dehydrogenases
AssigneeCeres, Inc.
InventorTimothy Swaller
Life of this patent
Estimating the term…
Abstract
The invention relates to nucleic acid and amino acid sequences for sorghum CAD alleles and truncated CAD polypeptides. Sorghum plants having such truncations or combinations thereof, methods of genotyping sorghum plants for CAD truncations, and methods for breeding sorghum plants having truncated CAD sequences or combinations thereof are described.
Claims14
An isolated nucleic acid comprising a nucleotide sequence encoding a CAD polypeptide, wherein said CAD polypeptide has at least 98% sequence identity to amino acids 1-130 or 1-319 of SEQ ID NO:
- 6 and terminates at a position corresponding to residue 131 or 320 of SEQ ID NO:
- 6.
An isolated nucleic acid comprising a nucleotide sequence encoding a sorghum CAD polypeptide, wherein said sorghum CAD polypeptide has at least 80% sequence identity to amino acids 1-130 or 1-319 of SEQ ID NO:
- 6 and terminates at a position corresponding to residue 131 or 320 of SEQ ID NO:
- 6.
A method of characterizing a sorghum plant comprising detecting a nucleic acid encoding a CAD polypeptide in said sorghum plant, wherein said CAD polypeptide has at least 80% sequence identity to amino acids 1-130 or 1-319 of SEQ ID NO:
- 6 and terminates at a position corresponding to residue 131 or 320 of SEQ ID NO:
- 6.
A method of determining the presence of a polynucleotide in a sorghum plant, comprising:
- a) contacting at least one probe or primer pair with nucleic acid from said sorghum plant, wherein said probe or primer pair is specific for a polynucleotide that encodes a CAD polypeptide, wherein said CAD polypeptide has at least 80% sequence identity to amino acids 1-130 or 1-319 of SEQ ID NO:
- 6 and terminates at a position corresponding to residue 131 or 320 of SEQ ID NO:
- 6;
- and,
- b) determining whether or not said polynucleotide is present in said sorghum plant.
A kit for genotyping a sorghum biological sample, said kit comprising a primer pair that specifically amplifies, or a probe that specifically hybridizes to, a polynucleotide that encodes a CAD polypeptide having at least 80% sequence identity to amino acids 1-130 or 1-319 of SEQ ID NO:
- 6 and terminating at a position corresponding to residues 131 or 320 of SEQ ID NO:
- 6.
Dependencies are inferred from the claim text.