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US 8,298,791 B2Granted
Purine-derived substance-producing bacterium and a method for producing purine-derived substance
AssigneeAjinomoto Co., Inc.
InventorsKiyoshi Matsuno, Yukiko Mori, Takayuki Asahara
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Abstract
A purine-derived substance is produced by culturing a Bacillus bacterium which has an ability to produce a purine-derived substance and has enhanced activity of an enzyme of the oxidative pentosephosphate pathway. The purine-derived substance is produced in the medium or the bacterial cells, and can be collected from the medium or the bacterial cells.
Claims16
A method for producing a purine-derived substance comprising:
- A) culturing a Bacillus bacterium in a medium, and
- B) collecting said purine-derived substance,
- wherein said Bacillus bacterium is able to produce a purine-derived substance and has been modified to increase ribose-5-phosphate isomerase activity by increasing the copy number of a gene encoding the ribose-5-phosphate isomerase or modifying an expression control sequence of the gene encoding the ribose-5-phosphate isomerase, wherein said ribose-5-phosphate isomerase is selected from the group consisting of:
- (A) a protein comprising the amino acid sequence of SEQ ID NO:
- 50;
- and
- (B) a modified protein comprising the amino acid sequence of SEQ ID NO:
- 50 wherein one to 10 amino acids are substituted, deleted, inserted, or added, and said modified protein has ribose-5-phosphate isomerase activity.
5 more elements
Dependencies are inferred from the claim text.