US 8,298,789 B2 · Orthogonal process for purification of recombinant human parathyroid hormone (rhPTH) (1-34)

Abstract

The present invention discloses a process for the preparation of rhPTH (1-34) also known as teriparatide by construction of a novel nucleotide, as an NcoI.IXhoI fragment as set forth in SEQ. ID. No.:1 encoding a chimeric fusion protein as set forth in SEQ.ID. No.:2 comprising of a fusion partner consisting of 41 amino acids belonging to Escherichia coli β-galactosidase (LacZ) gene, an endopeptidase cleavage site, rhPTH (1-34) gene fragment, cloning the said nucleotide in an expression vector under the control of T7 promoter, transforming Escherichia coli with the said vector and expressing the chimeric fusion protein in fed batch fermentation. The present invention further discloses a low feed rate lactose induction for optimized expression of rhPTH (1-34) in Escherichia coli. The present invention also discloses an unique, novel two step orthogonal purification process for rhPTH (1-34) comprising of cation exchange chromatography optionally followed by preparative chromatography selected from HIC or RP-HPLC to yield a target protein of ≧99% purity. The present invention discloses a simple, cost-effective, environmentally benign method of producing high purity rhPTH (1-34).

Claims5

Dependent claims

A process for synthesis of rhPTH (1-34), said process comprising:

i) constructing a cDNA coding chimeric nucleotide ORF as set forth in SEQ. ID NO.:
1 by amplification of the cDNA by RT-PCR using gene specific primers SEQ. ID NO.:
3, SEQ. ID NO.:
4, SEQ. ID NO.:
5, SEQ. ID NO.:
6, SEQ. ID NO.:
7 and SEQ. ID NO.:
8 encoding rhPTH (1-34) chimeric fusion protein as set forth in SEQ. ID NO.:
2,
ii) expressing the chimeric fusion protein in the presence of lactose as inducer in the range of about 10-30% of total protein,
iii) purifying the rhPTH (1-34) obtained by an orthogonal process wherein the purification is carried out by cation exchange chromatography to a purity of >98% optionally followed by hydrophobic interaction chromatography (HIC) or RP-HPLC to a purity of ≧99%.

8 more elements

Dependencies are inferred from the claim text.

Orthogonal process for purification of recombinant human parathyroid hormone (rhPTH) (1-34)

Life of this patent

Orthogonal process for purification of recombinant human parathyroid hormone (rhPTH) (1-34)

Life of this patent

Abstract

Claims5