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US 8,298,785 B2Granted
Use of ribozymes in the detection of adventitious agents
AssigneeOncolytics Biotech Inc.
InventorMatthew C. Coffey
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Abstract
The present invention provides a method of detecting adventitious agents in a composition comprising a microorganism by using ribozyme-expressing indicator cells, as well as indicator cells useful in such detection.
Claims10
A method of validating a therapeutic composition comprising reovirus as having no detectable adventitious agent, the method comprising:
- (a) providing a therapeutic composition in need of validation, the therapeutic composition comprising a reovirus and suspected of further comprising an adventitious agent, wherein the adventitious agent is not a reovirus;
- (b) providing a population of indicator cells that express a ribozyme, wherein the ribozyme is capable of specifically cleaving the genome of the reovirus;
- (c) contacting the indicator cells with the therapeutic composition under conditions that allow for cleavage of the genome of the reovirus by the ribozyme;
- and
- (d) determining the effect of any adventitious agent in the therapeutic composition by detecting the presence or absence of a pathogenic effect on the indicator cells contacted with the therapeutic composition, wherein the presence of a pathogenic effect indicates the presence of an adventitious agent in the therapeutic composition;
- and
- (e) validating the therapeutic composition as having no detectable adventitious agent in the absence of a pathogenic effect.
4 more elements
Dependencies are inferred from the claim text.