Method for diagnosis of disease using quantitative monitoring of protein tyrosine phosphatase
AssigneeKorea Research Institute of Bioscience and Biotechnology
InventorsSeong Eon Ryu, Dae Gwin Jeong, Tae Sung Yoon, Jeong Hee Moon, Seok-II Hong, Young Joon Hong
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Abstract
The present invention relates to a method for quantifying protein tyrosine phosphatase (referred as PTP hereinafter) in biosamples, precisely a diagnostic method for disease by quantifying PTP using mass spectrometry and profiling of comparative PTP levels. By quantifying PTP in biosamples and profiling thereof according to the method of the present invention, disease can be diagnosed and diverse disease conditions and health conditions can be confirmed via profiling.
Claims20
A method for quantification of protein tyrosine phosphatase (PTP) comprising the following steps:
- 1) hydrolyzing a sample separated from a test subject;
- 2) adding a known amount of an isotope-substituted synthetic standard peptide to the hydrolyzed sample of step 1), wherein the synthetic standard peptide is selected from SEQ ID NOs:191, 220, 225, 236, 246, 202, 231, 186, 172, 176 and 238;
- 3) extracting wild type peptide and the isotope-substituted synthetic standard peptide from the hydrolyzed sample of step 2), followed by quantitative analysis;
- and
- 4) comparing the levels of the wild type peptide and the isotope-substituted synthetic standard peptide of step 3) to calculate a quantity of the wild type peptide expression.
A method for quantification of protein tyrosine phosphatase (PTP) comprising the following steps:
- 1) concentrating PTP in a sample separated from a test subject;
- 2) hydrolyzing the concentrated sample of step 1);
- 3) adding a known amount of an isotope-substituted synthetic standard peptide to the hydrolyzed sample of step 2), wherein the synthetic standard peptide is selected from SEQ ID NOs:191, 220, 225, 236, 246, 202, 231, 186, 172, 176 and 238;
- and
- 4) comparing the levels of wild type peptide and the isotope-substituted synthetic standard peptide of step 3) to calculate a quantity of the wild type peptide expression.
A screening method of a colon cancer, liver cancer, or lung cancer related biomarker comprising the following steps:
- 1) hydrolyzing a sample separated from a subject with colon cancer, liver cancer, or lung cancer;
- 2) adding a known amount of an isotope-substituted synthetic standard peptide to the hydrolyzed sample of step 1), wherein the synthetic standard peptide is selected from SEQ ID NOs:191, 220, 225, 236, 246, 202, 231, 186, 172, 176 and 238;
- 3) extracting the wild type peptide and the isotope-substituted synthetic standard peptide from the hydrolyzed sample of step 2), followed by quantitative analysis thereof;
- 4) comparing the levels of a wild type peptide and the isotope-substituted synthetic standard peptide of step 3) to calculate a quantity of the wild type peptide expression;
- and
- 5) comparing the quantity of the wild type peptide of step 4) and the quantity of the wild type peptide extracted from a normal subject to confirm the standard peptide demonstrating a significant difference.
Dependencies are inferred from the claim text.