Rapid diagnosis method specific to avian influenza virus
AssigneesOsaka Prefectural Government, Sysmex Corporation
InventorsKazuo Takahashi, Yoshinobu Okuno, Hiroshi Nishimura, Takeshi Imoarai, Noriyuki Saito, Tomokuni Taniguchi
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Abstract
The present invention relates to a method for detecting an avian influenza virus by an immunological assay using an anti-influenza virus antibody being unreactive to human influenza type-A virus subtypes H1, H2 and H3 and a human influenza type-B virus and being reactive to plural subtypes of avian influenza viruses, and an immunochromatographic test tool for use in the method. According to the present invention, an avian influenza virus can be detected specifically, rapidly and in a simple manner, as distinguishing an avian influenza virus from a human influenza virus.
Claims10
A method for detecting an avian influenza virus, comprising the step of detecting an avian influenza virus in a sample by an immunological assay, with the use of an anti-influenza virus monoclonal antibody produced by hybridoma 4E3 (Accession No. FERM BP-10904).
An immunochromatographic test tool for detecting an avian influenza virus in a sample by utilizing a primary antibody against an influenza virus and a second antibody against an influenza virus, comprising:
- a sample addition member to which the sample is applied;
- a label holding member in which a primary antibody labeled with labeling substance is hold;
- and
- an immunochromatographic membrane carrier having a judgment region placed thereon, said judgment region having the secondary antibody immobilized thereon;
- wherein said primary antibody is an anti-influenza virus antibody being reactive to an avian influenza virus, and wherein said secondary antibody is an anti-influenza virus monoclonal antibody produced by hybridoma 4E3 (accession no. FERM BP-10904).
Dependencies are inferred from the claim text.