Ophthalmic emulsions containing an immunosuppressive agent

An ophthalmic oil-in-water emulsion, which comprises colloid particles having an oily core surrounded by an interfacial film, said emulsion comprising:

• 0.05 to 0.3% cyclosporine in weight to the total weight (w/w) of the emulsion,
• 0.5 to 4% w/w medium-chain triglycerides,
• 0.02% w/w benzalkonium chloride, and
• surfactants,
• wherein the surfactants consist of a mixture of tyloxapol and poloxamer,
• wherein the tyloxapol is present in an amount of 0.3% w/w and the poloxamer is present in an amount of 0.1% w/w, and
• wherein said emulsion is cationic,
• wherein said emulsion meets zeta potential stability under thermal stress conditions as determined by Test A:
• measuring zeta potential, in mV, of said emulsion at the time of preparation is Z0,
• sealing 5-10 ml of said emulsion in 10 ml Type I glass vials under nitrogen atmosphere without bubbling,
• storing said sealed vials at 80° C. for 7 days,
• measuring zeta potential, in mV, of said emulsion at 7 days, Z7days,
• determining the difference between Z7days−Z0, δA, the emulsion meeting the zeta potential stability under thermal stress conditions according to Test A for a δA less than the 10 mV standard error of zeta potential measurement,
• wherein said emulsion does not comprise phospholipids, and
• wherein said emulsion does not comprise substances capable of generating a negative charge.