Compositions and method for epigenetic modification of nucleic acid sequences in vivo
AssigneeCellcentric GmbH & Co. KG
InventorsWolf Reik, Hugh Morgan, Chun-Fung Chan
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Abstract
Demethylation of a methylated DNA sequence in a eukaryotic cell is described, utilizing a molecule that includes at least a first domain that exhibits a cytidine deaminase activity and at least a second domain that confers either a specific or non-specific DNA binding activity. The molecules of the invention are useful in somatic cell nuclear transfer and also in cancer therapy.
Claims11
An isolated polypeptide molecule capable of initiating a demethylation of a methylated DNA sequence in a eukaryotic cell, the molecule consisting of a first domain that exhibits a cytidine deaminase activity, wherein the first domain consists of the AidΔNES sequence encoded by SEQ ID NO:1, and a second domain that confers a DNA binding activity.
Dependencies are inferred from the claim text.