Reagent delivery system
AssigneeThe Secretary of State for Defence
InventorDavid James Squirrell
Life of this patent
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Abstract
A delivery apparatus for selectively delivering one or more liquid reagents into a reaction or test chamber (2), especially of an assay apparatus, the apparatus comprising: one or more respective storage chambers (5,6) for containing the one or more liquid reagents and arranged generally above the reaction or test chamber (2); and a plunger element (4) arranged and operable for insertion into the mouth of a selected storage chamber so as to displace a selected reagent from therewithin into the reaction or test chamber (2) generally therebelow by gravitational liquid overflow from the mouth of the chamber. The apparatus may conveniently be provided as a discrete delivery unit, with the storage chambers (5,6) prefilled with the selected reagents.
Claims45
A reaction, test or assay apparatus for the extraction, purification, and amplification of DNA comprising:
- a support unit, comprising:
- (i) one or more chambers containing one or more reagents useful in the extraction, purification and amplification of DNA, at least one of said one or more chambers being supplied with heating and cooling means;
- (ii) a hollow rod for accommodating a retractable magnet;
- and
- (iii) a separate receptacle having a diameter less than the one or more chambers the separate receptacle containing the hollow rod and dimensioned to accommodate the hollow rod in an upright orientation.
A thermal cycling apparatus, comprising:
- a support unit, comprising:
- (i) one or more chambers containing one or more reagents useful in the extraction, purification and amplification of DNA, at least one of said one or more chambers being supplied with an electrically conducting polymer to heat the contents of the chamber by passing a controlled electric current through it;
- (ii) a hollow rod for accommodating a retractable magnet;
- and
- (iii) a receptacle having a diameter less than the one or more chambers, the receptacle dimensioned to contain and accommodate the hollow rod, such that the hollow rod is maintained in an upright orientation.
A reaction, test or assay apparatus for the extraction and purification of DNA comprising:
- a support unit, comprising:
- (i) one or more chambers, at least one of said one or more chambers being supplied with heating and cooling means, wherein the heating means comprises an electrically conducting polymer to heat the contents of the chamber by passing a controlled electric current through it;
- (ii) a cylindrical hollow rod for accommodating a retractable magnet, the cylindrical rod being of constant external diameter along its entire length;
- and
- (iii) a receptacle having a diameter less than the one or more chambers, the receptacle dimensioned to contain and accommodate the constant external diameter of the hollow rod such that the hollow rod is maintained in an upright orientation.
Dependencies are inferred from the claim text.