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US 8,293,498 B2Granted
System for generation of viable reovirus from cloned cDNA
AssigneeVanderbilt University
InventorsTerence S. Dermody, Takeshi Kobayashi, James D. Chappell
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Abstract
The present invention provides methods for the generation of viable reoviruses using only cloned nucleic acid segments representing the RNA segments of the reovirus genome.
Claims16
A method of generating an infectious Reoviridae particle comprising:
- (a) providing cDNAs corresponding to each viral RNA transcript, each of said cDNAs being under the control of an RNA polymerase promoter;
- (b) transferring each of said cDNAs into a host cell that expresses said RNA polymerase that directs transcription from said promoter;
- and
- (c) culturing said host cell under conditions supporting production of a Reoviridae particle.
1 more element
Dependencies are inferred from the claim text.