Compositions and methods for producing isoprene
AssigneesDanisco US Inc., The Goodyear Tire & Rubber Company
InventorsMarguerite A. Cervin, Gopal K. Chotani, Frank J. Feher, Richard La Duca, Joseph C. McAuliffe, Andrei Miasnikov, Caroline M. Peres, Aaron S. Puhala, Karl J. Sanford, Fernando Valle, Gregory M. Whited
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Abstract
The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.
Claims86
Recombinant bacterial cells capable of increased production of isoprene, the cells comprising nucleic acids encoding:
- (a) an isoprene synthase polypeptide, wherein the isoprene synthase polypeptide is encoded by a heterologous nucleic acid,
- (b) an isopentenyl-diphosphate delta-isomerase (IDI) polypeptide, and at least one of a 1-Deoxyxylulose-5-phosphate synthase (DXS) polypeptide and/or
- one or more mevalonate (MVA) pathway polypeptides,
- wherein the cells produce greater than 400 nmole/gwcm/hr of isoprene.
Recombinant bacterial cells capable of increased production of isoprene, the cells comprising nucleic acids encoding:
- (a) an isoprene synthase polypeptide, wherein the isoprene synthase polypeptide is encoded by a heterologous nucleic acid,
- (b) an isopentenyl-diphosphate delta-isomerase (IDI) polypeptide, and at least one of a 1-Deoxyxylulose-5-phosphate synthase (DXS) polypeptide and/or
- one or more mevalonate (MVA) pathway polypeptides,
- wherein the cells have an average volumetric productivity of isoprene greater than 0.1 mg/Lbroth/hr.
Recombinant bacterial cells capable of increased production of isoprene, the cells comprising nucleic acids encoding:
- (a) an isoprene synthase polypeptide, wherein the isoprene synthase polypeptide is encoded by a heterologous nucleic acid,
- (b) an isopentenyl-diphosphate delta-isomerase (IDI) polypeptide, and at least one of a 1-Deoxyxylulose-5-phosphate synthase (DXS) polypeptide and/or
- one or more mevalonate (MVA) pathway polypeptides,
- wherein the cells produce at least 0.0037 grams of isoprene per gram of dry cell mass (gdcm).
Dependencies are inferred from the claim text.