Medium supplement for virus production
AssigneeAVIR Green Hills Biotechnology
InventorsElisabeth Röthl, Andrej Egorov
Life of this patent
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Abstract
The use of macrolide polyene antibiotics or derivatives or analogues thereof as culture supplement for the propagation of virus is described. Further pharmaceutical compositions comprising a virus and a macrolide polyene antibiotic or a derivative or analogue thereof and methods for using of macrolide polyene antibiotics for transfection and infection of cells as well as the use of macrolide polyene antibiotics for the isolation of virus from clinical samples are disclosed.
Claims14
A method of propagating influenza virus, comprising the step of adding amphotericin B to a cell culture comprising VERO cells and the influenza virus.
A method of transfecting Vero cells, comprising the step of adding amphotericin B to a cell culture comprising Vero cells before, simultaneously with or shortly after transfecting the Vero cells with expression plasmids containing viral RNA segments of influenza virus.
A method for the infection of cells for influenza virus cultivation using amphotericin B, comprising the following steps:
- a) infecting cells with at least one infectious influenza virus particle;
- b) adding at least amphotericin B to an inoculum or cultivation media together with trypsin to form a cell culture mixture;
- c) incubating the cells with the cell culture mixture;
- followed by
- d) harvesting of influenza viruses.
Dependencies are inferred from the claim text.