System and method for interpolative calibration
AssigneeApplied Biosystems, LLC
InventorStephen J. Gunstream
Life of this patent
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Abstract
Systems and methods are provided for calibrating emission data or other information signals collected during a polymerase chain reaction (PCR), amplification reaction, assay, process, or other reaction. Calibration of multiple detectable materials can be achieved during a single cycle or run, or during a plurality of runs of the reaction. A reading from every well, container, or other support region of a sample support does not have to be taken. Interpolation can be used to determine values for emission data or other information signals that were not taken, or are unknown, using detected emission data, or other detected information signals. By calibrating the detected emission data and the interpolated data, a more accurate reading of emission data or information signal can be obtained.
Claims18
A method of calibrating emission data for a sample support including a plurality of support regions, comprising:
- loading a detectable material into the plurality of support regions of a sample support, such that at least a first support region, but less than all, of the plurality of support regions contain the detectable material;
- receiving emission data values for the detectable material detected from the at least first support region of the plurality of support regions;
- identifying a second-support region of the plurality of support regions, that did not emit an emission data value for the detectable material, wherein the second support region is independent from the first support region;
- interpolating, based on the received emission data values, an emission data value for the received emission data values for the second support region;
- and
- calibrating the emission data based at least in part on the interpolated emission data value.
A method of calibrating emission data for a sample support including a plurality of support regions, comprising:
- loading a first detectable material into the plurality of support regions of a sample support such that at least a first support region, but less than all, of the plurality of support regions contains the first detectable material;
- loading a second detectable material into the plurality of support regions such that at least a second support region, but less than all, of the plurality of support regions contains the second detectable material;
- receiving emission data values for at least one of the first and second detectable materials detected from the first and second support regions;
- identifying at least one support region of the plurality of support regions that did not emit an emission data value for at least one of the first and second detectable materials, wherein the at least one support region is independent from the first and second support regions;
- interpolating, based on the received emission data values, an emission data value for the received emission data values for the at least one support region;
- and
- calibrating the emission data based at least in part on the interpolated emission data value.
A method of calibrating signal information for a sample support, comprising:
- loading a first detectable material into the plurality of support regions of a sample support such that a first support region, but less than all, of the plurality of support regions contains the first detectable material;
- loading at least a second detectable material into the plurality of support regions such that a second support region, but less than all, of the plurality of support regions contains the second detectable material;
- receiving signal information values for at least one of the first and second detectable materials detected from at least the first and second support regions;
- identifying at least one support region of the plurality of support regions, where signal information was not detected for at least one of the first and second detectable materials, wherein the at least one support region is independent from the at least the first and second support regions;
- and
- interpolating, based on the received signal information values, signal information for the received signal information values for the at least one support region;
- and
- calibrating the emission data based at least in part on the interpolated signal information.
Dependencies are inferred from the claim text.