Method and device for acquiring signals in laser scanning microscopy

Method for acquiring signals in laser scanning microscopy, including stages of:

• relative movement of a focused optical excitation beam (2) and an object (7) to be measured so that the focal point of said beam (2) runs along at least one predetermined path (26) in the volume of said object (7), and
• acquisition of optical measurement signals along said path (26) according to at least one acquisition parameter,
• characterised in that
• the path (26) of the excitation beam is determined so that the variations in the optical properties of at least one portion of the media passed through by said excitation beam (2) affecting the propagation of said excitation beam are substantially minimised between said acquisitions of optical measurement signals, and
• in that at least one acquisition parameter among said acquisition parameter(s) is modulated during the movement of said excitation beam (2).