US 8,283,640 B2 · Fluorescence detection method

Abstract

A fluorescence detection method allows fluorescence intensity detection from a fluorescently-labeled biological sample with high accuracy in shorter time, even when a fluorescence intensity from a microchip itself acting as background. A fluorescence intensity from an equivalent microchip, which is equivalent to a microchip with the fluorescently-labeled biological sample, is detected for a period from the start of application of excitation light until the intensity sufficiently attenuates, and a temporal change of the intensity is stored. The light is applied to the microchip and the sample is fed before the fluorescence intensity from the microchip sufficiently attenuates to detect the fluorescence intensity for a period from the start of application of the light to a point after the sample is fed. Values of the temporal change are subtracted from the intensity detected from the microchip with the sample to detect an intensity of fluorescence emitted from the sample.

Claims8

Dependent claims

A fluorescence detection method for detecting a fluorescence intensity of fluorescence emitted when excitation light is applied to a microchip with a fluorescently-labeled biological sample fed thereto, the method comprising:

detecting a fluorescence intensity of fluorescence emitted from an equivalent microchip itself for a period from the start of application of the excitation light until the fluorescence intensity sufficiently attenuates, the equivalent microchip being equivalent to the microchip;
storing a temporal change of the detected fluorescence intensity;
applying the excitation light to the microchip and feeding the biological sample to the microchip before a fluorescence intensity of fluorescence emitted from the microchip itself sufficiently attenuates;
detecting the fluorescence intensity of the fluorescence emitted from the microchip for a period from the start of application of the excitation light to a point of time after the biological sample is fed to the microchip;
and
subtracting, from the fluorescence intensity detected from the microchip with the biological sample fed thereto, values of the stored temporal change of the fluorescence intensity of the fluorescence emitted from the equivalent microchip itself until the fluorescence intensity sufficiently attenuates, whereby detecting a fluorescence intensity of the fluorescence emitted from the biological sample exposed to the excitation light.

3 more elements

applying the excitation light to the microchip and feeding the biological sample to the microchip before a fluorescence intensity of fluorescence emitted from the microchip itself sufficiently attenuates;
detecting the fluorescence intensity of the fluorescence emitted from the microchip for a period from the start of application of the excitation light to a point of time after the biological sample is fed to the microchip;
estimating a temporal change of the fluorescence intensity of the fluorescence emitted from the microchip for a period until the fluorescence intensity sufficiently attenuates, based on an initial temporal change of the fluorescence intensity of the fluorescence emitted from the microchip obtained through the detection;
and
subtracting, from the fluorescence intensity detected from the microchip with the biological sample fed thereto, values of the estimated temporal change of the fluorescence intensity of the fluorescence emitted from the microchip for the period until the fluorescence intensity sufficiently attenuates, whereby detecting a fluorescence intensity of the fluorescence emitted from the biological sample exposed to the excitation light.

2 more elements

Dependencies are inferred from the claim text.

Fluorescence detection method

Life of this patent

Fluorescence detection method

Life of this patent

Abstract

Claims8