Administration of transposon-based vectors to reproductive organs
AssigneesTransGenRx, Inc., Board of Supervisors of Louisiana State University and Agricultural and Mechanical College
InventorsRichard K. Cooper, William C. Fioretti, Gary G. Cadd
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Abstract
Methods and compositions for the administration of transposon-based vectors to the reproductive organs of animals and the creation of transgenic animals. Preferred methods involve administration of the transposon-based vectors to the lumen of the oviduct of an avian, expression of a vector derived transgene in the avian, and deposition of the resultant polypeptide in an egg. This invention allows for large amounts of protein to be deposited in the egg.
Claims17
A method of producing a transgenic chicken or quail comprising, administering to an artery leading to the ovary of a chicken or quail a composition comprising a transposon-based vector, wherein the transposon-based vector comprises a prokaryotic transposase gene operably linked to a first promoter, a modified Kozak sequence positioned 3′ to the first promoter so as to encode for at least the first codon of the transposase gene, and one or more genes of interest operably-linked to one or more additional promoters, wherein a plurality of the first twenty codons of the transposase gene are modified from the wild-type sequence by changing the nucleotide at the third base position of the codon to an adenine or thymine without modifying the amino acid encoded by the codon, and wherein the one or more genes of interest and their operably-linked promoters are flanked by transposase insertion sequences recognized by a transposase encoded by the transposase gene, such that the one or more genes of interest and their operably linked promoters and transposase insertion sequences are incorporated into a germ-line cell of the chicken or quail.
Dependencies are inferred from the claim text.