Method for purifying protein
AssigneeHiroshima University
InventorsAkio Kuroda, Takeshi Ikeda
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Abstract
The present invention provides a method for purifying a protein, includes the step of: contacting a fusion protein of a first protein and a second protein with a bivalent cation-containing solution, the fusion protein being adsorbed to a silicon oxide-containing substance, the first protein being capable of binding to the silicon oxide-containing substance in a solution containing 0.1M sodium chloride. With this arrangement, it is possible to easily produce large quantity of proteins which are high in purity without sacrificing activity of the proteins.
Claims9
A method for purifying a protein, comprising the step of:
- contacting a fusion protein of a first protein and a second protein with a bivalent cation-containing solution, the fusion protein being adsorbed to the silicon oxide component of a silicon oxide-containing substance, the first protein being capable of binding to the silicon oxide-containing substance in a solution containing 0.1 M sodium chloride, wherein the fusion protein dissociates from the silicon oxide-containing substance after the fusion protein is contacted with the bivalent cation-containing solution.
A method for purifying a protein, comprising the step of:
- contacting a protein adsorbed to the silicon oxide component of a silicon oxide-containing substance with a bivalent cation-containing solution,
- the protein being capable of binding to the silicon oxide-containing substance in a solution containing 0.1M sodium chloride, wherein the protein dissociates from the silicon oxide-containing substance after the protein is contacted with the bivalent cation-containing solution.
Dependencies are inferred from the claim text.