Recombinant fragments and synthetic peptides of 17-kDa polypeptide useful in detecting Bartonella henselae
AssigneeMedical Diagnostic Laboratories, LLC
InventorsLisa P. Huang, John G. Hoey, Martin E. Adelson, Eli Mordechai
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Abstract
The present disclosure describes recombinant and synthetic polypeptides of Bartonella henselae and related methods and kits for the detection of antibodies specific for Bartonella henselae. The 17-kDa polypeptides, methods and kits are useful in the detection of recent and/or ongoing infections with Bartonella henselae, which can be useful in the diagnosis of Cat Scratch Disease (CSD).
Claims10
A synthetic polypeptide consisting of the amino acid sequence SEQ ID NO:
- 22, whereby said synthetic polypeptide, when assayed in an ELISA assay, reacts to IFA sero-positive sera and does not react to IFA sero-negative sera from a patient infected with Bartonella henselae.
A synthetic polypeptide consisting of an amino acid sequence selected from the group consisting of SEQ ID NO:
- 24, SEQ ID NO:
- 25, SEQ ID NO:
- 26 and SEQ ID NO:
- 27, whereby said synthetic polypeptide, when assayed in an ELISA assay, reacts to IFA sero-positive sera and does not react to IFA sero-negative sera from a patient infected with Bartonella henselae.
A synthetic polypeptide consisting of the amino acid sequence SEQ ID NO:
- 32, whereby said synthetic polypeptide, when assayed in an ELISA assay, reacts to IFA sero-positive sera and does not react to IFA sero-negative sera from a patient infected with Bartonella henselae.
A synthetic polypeptide consisting of an amino acid sequence selected from the group consisting of SEQ ID NO:
- 30 and SEQ ID NO:
- 31, whereby said synthetic polypeptide, when assayed in an ELISA assay, reacts to IFA sero-positive sera and does not react to IFA sero-negative sera from a patient infected with Bartonella henselae.
A method for detection of Bartonella henselae in a biological sample from a mammal, comprising the steps of:
- (a) immobilizing a synthetic polypeptide to a solid support, wherein said synthetic polypeptide consisting of an amino acid sequence selected from the group consisting of SEQ ID NO:
- 22, SEQ ID NO:
- 24, SEQ ID NO:
- 25, SEQ ID NO:
- 26, SEQ ID NO:
- 27, SEQ ID NO:
- 30, SEQ ID NO:
- 31, and SEQ ID NO. 32;
- (b) adding a biological sample onto said support to permit antibodies present in said biological sample to bind to said immobilized synthetic polypeptide;
- (c) washing to remove any unbound antibodies;
- and
- (d) detecting bound antibodies, wherein the presence of said bound antibodies is indicative of infection of said mammal with Bartonella hensela.
Dependencies are inferred from the claim text.