Immunostimulatory nucleic acids
AssigneesColey Pharmaceutical Group, Inc., Coley Pharmaceutical GmbH
InventorsArthur M. Krieg, Grayson Lipford, Ulrike Samulowitz, Jörg Vollmer, Eugen Uhlmann, Marion Jurk, Robert Rankin
Life of this patent
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Abstract
The invention relates to a class of soft or semi-soft CpG immunostimulatory oligonucleotides that are useful for stimulating an immune response.
Claims11
An oligonucleotide having the following structure:
- (SEQ ID NO:
- 313)
- 5′ T*C_G*T*C_G*T*T*T*T*G*A*C_G*T*T*T*T*G*T*C_G*T*
- T 3′,
- wherein * refers to the presence of a phosphorothioate internucleotide linkage, and wherein _ refers to the presence of a phosphodiester internucleotide linkage and wherein the oligonucleotide has a length of 24-40 nucleotides.
An oligonucleotide having the following structure:
- (SEQ ID NO:
- 313)
- 5′ T*C_G*T*C_G*T*T*T*T*G*A*C_G*T*T*T*T*G*T*C_G*T*
- T 3′
- wherein each * refers to a phosphorothioate internucleotide linkage and each _ refers to a phosphodiester internucleotide linkage, and wherein the oligonucleotide is 24 nucleotides in length.
An immunostimulatory oligonucleotide having at least two internal cytosine-guanine (CG) dinucleotides, wherein C is unmethylated cytidine, and G is guanosine, wherein the at least two internal CG dinucleotides have phosphodiester internucleotide linkages, wherein optionally each additional internal CG dinucleotide has a phosphodiester or stabilized internucleotide linkage, wherein all other internucleotide linkages are stabilized with a phosphorothioate internucleotide linkage, and wherein the immunostimulatory oligonucleotide is 6-100 nucleotides long.
Dependencies are inferred from the claim text.