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US 8,283,183 B2Granted
Detecting method, detection device and detection kit
AssigneeCanon Kabushiki Kaisha
InventorsTakashi Ikeda, Norihiko Utsunomiya, Junta Yamamichi
Life of this patent
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Abstract
A detection device and a detecting method using the detection device are provided in which a magnetic particle is used as a marker particle, and the ratio of a region with reversed magnetization to the whole area of a free layer of a magnetoresistive effect film is increased by a stray magnetic field generated through a biochemical reaction from the magnetic particle remaining on a surface of the magnetoresistive effect film, so that a large detection signal is obtained and obtained detection data can be stored with stability.
Claims10
A detection device for detecting a magnetic particle in a sample solution, the device comprising:
- a magnetoresistive effect film including a first magnetic film with a fixed magnetization direction and a second magnetic film serving as a perpendicularly magnetized film with a magnetization direction variable at the detection of the magnetic particle;
- and
- a fixation layer for fixing the magnetic particle arranged on a surface of the second magnetic film,
- wherein all of the surface of the second magnetic film is set within a circle having a radius of 21/2d, where d represents a distance between the surface of the second magnetic film and a center of the magnetic particle, when the magnetic particle is fixed to the magnetoresistive effect film through the fixation layer,
- a direction of an external magnetic field for magnetizing the magnetic particle is parallel to a magnetization direction of the second magnetic film, and
- all of the second magnetic film is arranged in a region in which a stray magnetic field in the same direction as the external magnetic field is applied.
3 more elements
A detection device for detecting a magnetic particle in a sample solution, the device comprising:
- a magnetoresistive effect film including a first magnetic film with a fixed magnetization direction and a second magnetic film serving as an in-plane magnetized film with a magnetization direction variable at the detection of the magnetic particle;
- and
- a fixation layer for fixing the magnetic particle arranged on a surface of the second magnetic film,
- wherein all of the surface of the second magnetic film is set within a circle having a radius of d/21/2, where d represents a distance between the surface of the second magnetic film and a center of the magnetic particle, when the magnetic particle is fixed to the magnetoresistive effect film through the fixation layer,
- a direction of an external magnetic field for magnetizing the magnetic particle is parallel to a magnetization direction of the second magnetic film, and
- all of the second magnetic film is arranged in a region in which a stray magnetic field in an opposite direction from that in which the external magnetic field is applied.
3 more elements
A detection kit for detecting a target material in a specimen, the kit comprising at least:
- a sensing element including a magnetoresistive effect film having a first magnetic film with a fixed magnetization direction and a second magnetic film serving as a perpendicularly magnetized film with a magnetization direction variable at the detection of the magnetic particle;
- a fixation layer for fixing the magnetic particle arranged on a surface of the second magnetic film;
- a detection reagent including a magnetic particle on which a trap for trapping the target material is fixed,
- wherein all of the surface of the second magnetic film is set within a circle having a radius of 21/2d, where d represents a distance between the surface of the second magnetic film and a center of the magnetic particle, when the magnetic particle is fixed to the magnetoresistive effect film through the fixation layer,
- a direction of an external magnetic field for magnetizing the magnetic particle is parallel to a magnetization direction of the second magnetic film, and
- all of the second magnetic film is arranged in a region in which a stray magnetic field in the same direction as the external magnetic field is applied.
3 more elements
A detection kit for detecting a target material in a specimen, the kit comprising at least:
- a sensing element including a magnetoresistive effect film having a first magnetic film with a fixed magnetization direction and a second magnetic film serving as an in-plane magnetized film with a magnetization direction variable at the detection of the magnetic particle;
- a fixation layer for fixing the magnetic particle arranged on a surface of the second magnetic film;
- and
- a detection reagent including a magnetic particle on which a trap for trapping the target material is fixed,
- wherein all of the surface of the second magnetic film is set within a circle having a radius of d/21/2, where d represents a distance between the surface of the second magnetic film and a center of the magnetic particle, when the magnetic particle is fixed to the magnetoresistive effect film through the fixation layer,
- a direction of an external magnetic field for magnetizing the magnetic particle is parallel to a magnetization direction of the second magnetic film, and
- all of the second magnetic film is arranged in a region in which a stray magnetic field in an opposite direction from that in which the external magnetic field is applied.
4 more elements
Dependencies are inferred from the claim text.