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US 8,283,180 B2Granted
Periodic acid-schiff staining with detection in the infrared range
AssigneeAmicus Therapeutics, Inc.
InventorElfrida Benjamin
Life of this patent
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Abstract
Provided is a method of detecting the presence and quantitating the amount of glycogen from a biological sample. This method employs PAS staining with detection in the infrared range.
Claims11
A method for detecting polysaccharides in a biological sample, which method consists essentially of the sequential steps of
- subjecting the sample to Periodic Acid-Schiff staining to provide Periodic Acid-Schiff stained sample,
- washing the sample to remove unreacted Schiff's reagents followed by detecting light fluorescing from said Periodic Acid-Schiff stained sample at a wavelength in the range from about 700 nM to 800 nM;
- wherein the biological sample is isolated cells or tissue selected from the group consisting of liver, kidney, heart, brain and skeletal muscle.
A method for detecting glycogen in a biological sample from a subject having or suspected of having Pompe disease, which method consists essentially of the sequential steps of
- subjecting the sample to Periodic Acid-Schiff staining to provide Periodic Acid-Schiff stained sample,
- washing the sample to remove unreacted Schiff's reagents followed by detecting light fluorescing from said Periodic Acid-Schiff stained sample at a wavelength in the range from about 700 nM to 800 nM;
- wherein the biological sample is isolated cells or tissue selected from the group consisting of liver, kidney, heart, brain and skeletal muscle.
Dependencies are inferred from the claim text.