Mesoderm and definitive endoderm cell populations
AssigneeMount Sinai School of Medicine
InventorsGordon M. Keller, Paul Gadue
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Abstract
The present invention provides cell populations that are enriched for mesendoderm and mesoderm, and cell populations that are enriched for endoderm. The cell populations of the invention are useful for generating cells for cell replacement therapy. The present invention further provides a method of generating hepatocytes, cell populations enriched for hepatocytes, and a method of hepatocyte replacement therapy.
Claims8
A method of making a cell population enriched for hepatocytes comprising culturing embryonic stem cells in the presence of activin or nodal to provide brach+/HFN3β+/cKit+ cells, isolating the brach+/HFN3 β+/cKit+ cells, culturing said brach+/HFN3β+/cKit+ cells in the presence of BMP-4 and bFGF in serum-free media to produce hepatoblasts, and culturing the hepatoblasts in serum-free media supplemented with one or more cytokines that support hepatocyte growth for an amount of time sufficient to produce a population enriched for hepatocytes.
A method of making a cell population enriched for hepatocytes comprising culturing pluripotent stem cells in the presence of activin to provide brach+/HFN3 β+/cKit+ cells, culturing said brach+/HFN3β+/cKit+ cells in the presence of BMP-4 and bFGF in serum-free media to produce hepatoblasts, and culturing the hepatoblasts in serum-free media supplemented with one or more cytokines that support hepatocyte growth for an amount of time sufficient to produce a population enriched for hepatocytes.
Dependencies are inferred from the claim text.