Preparation of annexin derivatives
AssigneeClear Vascular, Inc.
InventorJaime Simon
Life of this patent
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Abstract
Labeled annexin is formed by reacting annexin or a modified annexin with a phosphatidylserine group or an analogue of phosphatidylserine. This phosphatidylserine annexin conjugate is then reacted with an appropriate label to form a labeled annexin and the annexin is separated from the phosphatidylserine group. The reaction between the phosphatidylserine group and the annexin is calcium ion concentration dependent. Therefore, the reaction can be promoted by having a high calcium ion concentration and the separation of the phosphatidyl group from the annexin group is facilitated by reducing the calcium ion concentration preferably by addition of an appropriate calcium chelating agent.
Claims8
A method of preparing annexin V bound payload compound comprising steps in the following order:
- binding said annexin V to phosphatidylserine to form a phosphatidylserine annexin V complex having the annexin V moiety and the phosphatidylserine moiety in the presence of concentration of calcium ions of at least 1 mM;
- binding said payload to said annexin V moiety of said phosphatidylserine annexin V complex;
- and
- separating said payload and said annexin V moiety from said phosphatidylserine moiety thereby to form said annexin V bound payload compound.
Dependencies are inferred from the claim text.