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US 8,283,149 B2Granted
Detergent composition comprising a polypeptide having lipase activity and polynucleotide encoding same
AssigneeNovozymes A/S
InventorsXiaoMei Niu, Ye Liu, Jurgen Carsten Franz Knotzel, Signe Munk
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Abstract
The present invention relates to a new wild-type lipase, its nucleic acid sequences and the amino acid sequences thereof. This new lipase shows washing performance in the form of wild-type. The present invention also relates to the method for producing the lipase and the uses of the lipase in baking.
Claims18
A detergent composition comprising an isolated polypeptide having lipase activity selected from the group consisting of:
- (a) a polypeptide comprising an amino acid sequence having at least 80% sequence identity to the mature polypeptide of SEQ ID NO:
- 2;
- (b) a polypeptide having an amino acid sequence which has at least 80% sequence identity with amino acids 1 to 315 of SEQ ID NO:
- 2;
- (c) a polypeptide encoded by a polynucleotide that hybridizes under at least high stringency conditions with (i) the complement of the mature polypeptide coding sequence of SEQ ID NO:
- 1, or (ii) the complement of the cDNA sequence contained in or the genomic DNA sequence comprising the mature polypeptide coding sequence of SEQ ID NO:
- 1;
- (d) a polypeptide which is encoded by a polynucleotide which hybridizes under at least high stringency conditions with (i) the complement of nucleotides 41 to 1069 of SEQ ID NO:
- 1, or (ii) the complement of the cDNA sequence contained in or the genomic DNA sequence comprising nucleotides 41 to 1069 of SEQ ID NO:
- 1;
- and
- (e) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 80% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 1;
- and wherein the high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5x SSPE, 0.3% SDS, 200 micrograms/mL sheared and denatured salmon sperm DNA and 50% formamide, and washing three times each for 15 minutes using 2x SSC and 0.2% SDS at 65° C.,
- and wherein the composition comprises a detergent ingredient.
12 more elements
Dependencies are inferred from the claim text.